Isplay an increase in gene expression more than time (Table 1, an heatmat showing gene expression values is reported in More file 2). Glucose metabolism, especially glycolysis, is represented by many genes displaying the same trend; aldolase a (P_contig00403), glucose phosphate isomerase b (N_isotig03674), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, N_isotig18841), lactate dehydrogenase (P_isotig00860) and quite a few other individuals improve in expression more than 20-fold from 1 to 33 dph. By contrast, genes integrated in the pathways “Hedgehog signalling” and “Wnt signalling” displayed decreasing expression more than time (see Table 1, Further file 2 for corresponding heatmaps). These two essential pathways are involved in developmental processes and manage of asymmetric cell division. In unique, a sizable quantity of genes associated to “Hedgehog signalling” displayed a decreasing temporal trend of expression, like sonic hedgehog-like (P_isotig18139), bone morphogenetic protein 7b (P_isotig17732), megalin (P_isotig07996), and hedgehog interacting protein (N_isotig19239). Likewise,N_isotig04075 N_isotig11618 N_isotig08672 N_isotig19778 N_isotig05817 P_isotig17511 N_isotig01855 N_isotig03727 P_isotig01165 P_isotig18441 N_isotig17357 Probe Name N_contig01740 N_isotig13675 N_isotig03674 P_isotig13251 P_contig00403 N_isotig03547 P_isotig10593 P_isotig18039 N_isotig03683 N_isotig04393 N_isotig06502 N_isotigGLUCOSE METABOLISMFerraresso et al. BMC Genomics 2013, 14:315 http://biomedcentral/1471-2164/14/Page five ofTable 1 Genes up- or down-regulated more than time (Continued)N_isotig18841 P_isotig00860 P_isotig00400 N_contig00167 N_isotig05258 N_isotig12521 N_isotig06792 P_isotig13923 P_isotig03551 N_isotig20169 ENSDARG00000039914 ENSDARG00000040856 ENSDARG00000043180 ENSDARG00000054191 ENSDARG00000057571 ENSDARG00000057630 ENSDARG00000060797 ENSDARG00000062998 ENSDARG00000070826 ENSDARG00000071076 Glyceraldehyde-3phosphate dehydrogenase Lactate dehydrogenase A4 Glycerol-3-phosphate dehydrogenase 1b Phosphoglycerate kinase 1 Phosphoglycerate mutase 2 (muscle) Aldose 1-epimerase Phosphofructokinase, muscle b Peptidoglycan recognition protein 2 2,3-bisphosphoglycerate mutase Similar to L-lactate dehydrogenase B chain Gene name GLI-Kruppel family member GLI3 Casein kinase 1, delta a Casein kinase 1, gamma 2a Glycogen synthase kinase three beta (GSK3B) Casein kinase 1, gamma 2b Bone morphogenetic protein five Wingless-type MMTV integration site family,7Bb (WNT7) Hedgehog interacting protein (HiP) F-box and WD-40 domain protein 11b (FBXW11) Similar to cAMP-dependent protein kinase (PKA Calpha) Protein kinase, cAMPdependent, catalytic, beta Megalin, low density lipoprotein-related protein two (LRP2) Casein kinase 1, alpha 1 Zic loved ones member 2 Bone morphogenetic protein 7b Sonic hedgehog-like; Sonic hedgehog aTable 1 Genes up- or down-regulated more than time (Continued)WNT SIGNALLING PATHWAY Probe Name P_isotig22061 P_isotig10261 Ensembl Acc.Formula of DABCO-Bis(sulfur dioxide) Quantity ENSDARG00000004305 ENSDARG00000007791 Gene name Vang-like 1 (van gogh, Drosophila) Protein phosphatase two (formerly 2A), regulatory subunit, beta Dishevelled connected activator of morphogenesis 1 Mitogen-activated protein kinase 8 Related to Casein kinase II subunit alpha (CK II) Similar to cAMP-dependent protein kinase (PKA Calpha) Catenin, beta two Calcyclin binding protein F-box and WD-40 domain protein 11b Glycogen synthase kinase 3 beta Cullin 1a Mitogen-activated protein kinase ten Vang-like two (van gogh, Drosophila) Lymphocyte enha.387859-70-3 custom synthesis PMID:26760947
